Composite

Part:BBa_K3286204:Design

Designed by: Ben Kuipers   Group: iGEM19_Wageningen_UR   (2019-10-20)


Two component system RpfCch + RpfG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1922
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1922
    Illegal BglII site found at 288
    Illegal BglII site found at 3362
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1922
    Illegal PstI site found at 887
    Illegal PstI site found at 1493
    Illegal PstI site found at 2120
    Illegal NgoMIV site found at 818
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1736
    Illegal NgoMIV site found at 1831
    Illegal NgoMIV site found at 2890
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For a rapid turn over of c-di-GMP, we wanted to have a high concentration of both RpfCch and RpfG. Therefore we used the strongest referenced Anderson promoter and the strongest RBS present in the iGEM repository.


Source

The source of this part are its components.

References