Composite
Part:BBa_K3286204:Design
Designed by: Ben Kuipers Group: iGEM19_Wageningen_UR (2019-10-20)
Two component system RpfCch + RpfG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1922
Illegal PstI site found at 887
Illegal PstI site found at 1493
Illegal PstI site found at 2120 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1922
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 887
Illegal PstI site found at 1493
Illegal PstI site found at 2120 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1922
Illegal BglII site found at 288
Illegal BglII site found at 3362 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1922
Illegal PstI site found at 887
Illegal PstI site found at 1493
Illegal PstI site found at 2120 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1922
Illegal PstI site found at 887
Illegal PstI site found at 1493
Illegal PstI site found at 2120
Illegal NgoMIV site found at 818
Illegal NgoMIV site found at 909
Illegal NgoMIV site found at 1736
Illegal NgoMIV site found at 1831
Illegal NgoMIV site found at 2890 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
For a rapid turn over of c-di-GMP, we wanted to have a high concentration of both RpfCch and RpfG. Therefore we used the strongest referenced Anderson promoter and the strongest RBS present in the iGEM repository.
Source
The source of this part are its components.